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traction force microscopy data analysis  (GraphPad Software Inc)
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GraphPad Software Inc traction force microscopy data analysis
R491 increases the fraction and number of small focal adhesions. (A) Representative confocal <t>microscopy</t> images of BJ-Ras cells treated with 5 μM control or R491, showing vimentin, phospho-tyrosine, F-actin, as indicated, and merged images with vimentin (red), phospho-tyrosine (yellow), actin (green) and nuclei (blue). Inset images: magnified views of boxed areas in the larger images. Scale bars: 10 μm. The graphs show (B) the average size of cell-matrix adhesions/per cell, with n = 516 and 478 focal adhesions for control- and R491-treated cells, respectively, and (C) the frequency distribution of the binned sizes of focal adhesions of control- (black) or R491- (grey) treated cells. ** p ≤ 0.01. The difference in the frequency distribution was significant with p ≤ 0.0001.
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R491 increases the fraction and number of small focal adhesions. (A) Representative confocal microscopy images of BJ-Ras cells treated with 5 μM control or R491, showing vimentin, phospho-tyrosine, F-actin, as indicated, and merged images with vimentin (red), phospho-tyrosine (yellow), actin (green) and nuclei (blue). Inset images: magnified views of boxed areas in the larger images. Scale bars: 10 μm. The graphs show (B) the average size of cell-matrix adhesions/per cell, with n = 516 and 478 focal adhesions for control- and R491-treated cells, respectively, and (C) the frequency distribution of the binned sizes of focal adhesions of control- (black) or R491- (grey) treated cells. ** p ≤ 0.01. The difference in the frequency distribution was significant with p ≤ 0.0001.

Journal: Frontiers in Cell and Developmental Biology

Article Title: ALD-R491 regulates vimentin filament stability and solubility, cell contractile force, cell migration speed and directionality

doi: 10.3389/fcell.2022.926283

Figure Lengend Snippet: R491 increases the fraction and number of small focal adhesions. (A) Representative confocal microscopy images of BJ-Ras cells treated with 5 μM control or R491, showing vimentin, phospho-tyrosine, F-actin, as indicated, and merged images with vimentin (red), phospho-tyrosine (yellow), actin (green) and nuclei (blue). Inset images: magnified views of boxed areas in the larger images. Scale bars: 10 μm. The graphs show (B) the average size of cell-matrix adhesions/per cell, with n = 516 and 478 focal adhesions for control- and R491-treated cells, respectively, and (C) the frequency distribution of the binned sizes of focal adhesions of control- (black) or R491- (grey) treated cells. ** p ≤ 0.01. The difference in the frequency distribution was significant with p ≤ 0.0001.

Article Snippet: The mean values of the aspect ratio, cell migration speed and persistence, cells from which debris is detached and left behind during migration, filament distribution, as well as Traction force microscopy data were analyzed using GraphPad Prism (v.9.3.1).

Techniques: Confocal Microscopy, Control